Establishing CRISPR/Cas9 in Lipomyces starkeyi

  • Zoe Lau WISEST student researcher, University of Alberta
  • David Stuart Department of Biochemistry, University of Alberta
  • Bonnie Mcneil Department of Biochemistry, University of Alberta
Keywords: CRISPR, Lipomyces starkeyi, gene editing, biofuels, Lipomyces sarkeyi biofuels

Abstract

The goal of this project was to adapt the Yarrowia lipolytica plasmid based CRISPR/Cas9 system for usage in Lipomyces starkeyi. Lipomyces starkeyi is an oleaginous yeast, which synthesizes and stores high amounts of intracellular lipids. This specific yeast can store lipids at concentrations higher than 60% of its dry cell weight. Due to these high concentrations of lipids, L. starkeyi is a desired organism for the production of biofuels and other oleochemicals. However, there is a lack of knowledge and of genetic tools when trying to engineer the cells to produce these lipids for our use. The genome editing tool, CRISPR/Cas9 is efficient and simple, therefore desirable for the engineering of L. starkeyi. The goal was achieved by replacing the Y. lipolytica promoter with a L. starkeyi promoter, inserting guide RNA, as well as confirming cas9 protein expression.

Published
2019-09-16